Expressing:Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Recombinant:Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Plasmid Preparation:Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Transfection:Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Suspension:Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 187), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of the CD3 gamma-epsilon-Fc protein (SEQ ID NO: 170), the recombinant vector was transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 gamma-epsilon-Fc construct was then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Construct:Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: CD3/CD38 T cell retargeting hetero-dimeric immunoglobulins and methods of their production
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 173 and 174, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
Article Title: Production of T cell retargeting hetero-dimeric immunoglobulins
Article Snippet: .. For transient expression of human and cynomolgus CD3 epsilon constructs (SEQ ID NO: 190 and 191, respectively), the recombinant vectors were transfected into suspension-adapted HEK-EBNA cells (ATCC®-CRL-10852) using Polyethyleneimine (PEI) as described above. .. The CD3 epsilon fusion constructs were then purified from cell-free supernatant using recombinant Streamline rProtein A media (GE Healthcare Europe GmbH, Glattbrugg, Switzerland) and used for further analysis.
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